Thanks for the responses! Much appreciated
All the tanks are the same size and have a Fluval 1 filter in.
The distilled water was concerning me too. Do you think it's a good
idea to just use tap water and treat it with a water hardness
adjuster? Can't get RO water here... well not with any ease anyway!
Tank 1 - is my control tank
Tank 2 - I'm not worried about as the food is there to breakdown and
produce the ammonia.
Tank 3 - I've read on these threads that in a lot of peoples pinions
that due to the lack of nitrospira that Cycle won't work at this stage
of tank cycling which, if it fails as a maturation method doesn't
concern me because then it just shows that it doesn't work. But it
won't work either way if there is no ammonia present or this is what I
gather from what you're saying... so I think I will use the fish food
like in tank 2, same amount.
Tank 4 - Again from your response I gather I need a source of ammonia
so I think I'll go for the food option... again same amount.
Have never seen BioSpira, Amquel or Prime over here so I don't know if
it's available. I worked in a big fish shop last year selling fish and
aquaria products and I never came across those. I do have a limited
knowledge of fish keeping but before I started work I had no knowledge
at all so I am still learning. As for trying a tank with fish - I
doubt my college would allow it to be honest! I want to do 7 max, 5
min repetitions of the experiments. The final dissertation is due in
May 5th and due to things like inspections (Ofsted etc), Christmas
holidays and half terms the amount of time I have to do it I is
getting shorter and shorter. Feeling a little out of my depth and I'm
extremely gratefully for you guys helping me!
I am cycling 7 days (I know its 7 to 10 but because of the time
constraints I am only doing 7 days). I am water testing daily for
ammonia, nitrates, nitrites and pH except for weekends when I don't
have access to the labs.
To the scientist - Ingrid.
My methods of counting the bacteria probably a bit rubbish!
- I have put 2 slides in each filter and at the end of cycling for 7
days I take them out and gram stain them and counting the amount of
bacteria through random fields of vision.
- At the end of the 7 day cycle sponges are removed, put into a jar of
distilled water and put in a shaker for x amount of time. The water is
then sampled and bacteria cells then counted using a haemocytometer.
The lab technician and I have been looking at ways to culture the
bacteria from the sponges but keep hitting a brick wall. We know it's
possible to culture but we can't find out exactly what the media is or
what exactly it's made up of.
Please excuse my ignorance but what do you mean by a small GF. Did you
Thanks again for all your help everyone. >> Stay informed about: Maturing fish tanks. Which is the best?